Polyethylene glycol-grafted poly-l-lysine as polymeric gene carrier
Identifieur interne : 002735 ( Main/Exploration ); précédent : 002734; suivant : 002736Polyethylene glycol-grafted poly-l-lysine as polymeric gene carrier
Auteurs : Young Hun Choi [Corée du Sud] ; Feng Liu [États-Unis] ; Jin-Seok Kim [États-Unis] ; Young Kweon Choi [États-Unis] ; Jong Sang Park [Corée du Sud] ; Sung Wan Kim [États-Unis]Source :
- Journal of Controlled Release [ 0168-3659 ] ; 1998.
English descriptors
- Teeft :
- Assay, Best transfection, Bioconjugate chem, Cell membranes, Cell viability, Chem, Chloroquine, Choi, Complete retardation, Cytotoxicity, Different types, Displacement assay, Dynamic laser light, Early gene expression, Early promoter, Embl accession, Endocytosis mechanism, Ethidium bromide, Excess thionyl chloride, Gene, Gene carriers, Gene delivery, Gene expression, Gene expression level, Gene transfer, Hepes buffer, High cytotoxicity, High solubility, Higher transfection, Highest transfection frequency, Human carcinoma cell line, Human gene therapy, Lipofectin, Lower cytotoxicity, Mammalian cells, Mole, Molecular weight marker, Nacl buffer, Nucleic acids, Plasmid, Polyethylene glycol, Polymer, Polymer complexes, Reaction conditions, Retardation assay, Retroviral vectors, Room temperature, Size marker, Thionyl chloride, Tissue culture incubator, Transfection, Transfection assay, Transfection experiments, Transfection mixture, Uorescence intensity, Various amounts, Viral vectors, Weight ratio.
Abstract
Abstract: A new series of gene carriers, polyethylene glycol (PEG)-grafted poly-l-lysine (PLL, mol. wt.=25 000) with three different PEG-grafted ratios (5, 10 and 25 mole%, which means 5, 10 and 25% of ϵ-amino group of PLL was modified by PEG), was synthesized. These new gene carriers, named comb-shaped PEG-g-PLL copolymer, showed a 5- to 30-fold increase in transfection efficiency compared to PLL alone on a human carcinoma cell line. It is likely that Hep G2 cells were transfected by plasmid DNA/PEG-g-PLL complexes through an endocytosis mechanism due to the fact that chloroquine increased transfection efficiency. Although Lipofectin™, a cationic lipid formulation, showed slightly higher transfection efficiency than PEG-g-PLL in Hep G2 cells, our designed PEG-g-PLL demonstrated lower cytotoxicity, early gene expression and maintenance of gene expression for up to 96 h.
Url:
DOI: 10.1016/S0168-3659(97)00174-0
Affiliations:
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type="state">Utah</region></placeName></affiliation></author><author><name sortKey="Kim, Jin Seok" sort="Kim, Jin Seok" uniqKey="Kim J" first="Jin-Seok" last="Kim">Jin-Seok Kim</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Center for Controlled Chemical Delivery, Department of Pharmaceutics and Pharmaceutical Chemistry, University of Utah, 570 Biomedical Polymers Research Building, Room 205, Salt Lake City, UT 84112</wicri:regionArea><placeName><region type="state">Utah</region></placeName></affiliation></author><author><name sortKey="Choi, Young Kweon" sort="Choi, Young Kweon" uniqKey="Choi Y" first="Young Kweon" last="Choi">Young Kweon Choi</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Center for Controlled Chemical Delivery, Department of Pharmaceutics and Pharmaceutical Chemistry, University of Utah, 570 Biomedical Polymers Research Building, Room 205, Salt Lake City, UT 84112</wicri:regionArea><placeName><region type="state">Utah</region></placeName></affiliation></author><author><name sortKey="Jong Sang Park" sort="Jong Sang Park" uniqKey="Jong Sang Park" first="" last="Jong Sang Park">Jong Sang Park</name><affiliation wicri:level="4"><country xml:lang="fr">Corée du Sud</country><wicri:regionArea>Department of Chemistry, Seoul National University, Seoul</wicri:regionArea><placeName><settlement type="city">Séoul</settlement><region type="capital">Région capitale de Séoul</region></placeName><orgName type="university">Université nationale de Séoul</orgName></affiliation></author><author><name sortKey="Kim, Sung Wan" sort="Kim, Sung Wan" uniqKey="Kim S" first="Sung Wan" last="Kim">Sung Wan Kim</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><wicri:regionArea>Center for Controlled Chemical Delivery, Department of Pharmaceutics and Pharmaceutical Chemistry, University of Utah, 570 Biomedical Polymers Research Building, Room 205, Salt Lake City, UT 84112</wicri:regionArea><placeName><region type="state">Utah</region></placeName></affiliation><affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Journal of Controlled Release</title><title level="j" type="abbrev">COREL</title><idno type="ISSN">0168-3659</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1998">1998</date><biblScope unit="volume">54</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="39">39</biblScope><biblScope unit="page" to="48">48</biblScope></imprint><idno type="ISSN">0168-3659</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0168-3659</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Assay</term><term>Best transfection</term><term>Bioconjugate chem</term><term>Cell membranes</term><term>Cell viability</term><term>Chem</term><term>Chloroquine</term><term>Choi</term><term>Complete retardation</term><term>Cytotoxicity</term><term>Different types</term><term>Displacement assay</term><term>Dynamic laser light</term><term>Early gene expression</term><term>Early promoter</term><term>Embl accession</term><term>Endocytosis mechanism</term><term>Ethidium bromide</term><term>Excess thionyl chloride</term><term>Gene</term><term>Gene carriers</term><term>Gene delivery</term><term>Gene expression</term><term>Gene expression level</term><term>Gene transfer</term><term>Hepes buffer</term><term>High cytotoxicity</term><term>High solubility</term><term>Higher transfection</term><term>Highest transfection frequency</term><term>Human carcinoma cell line</term><term>Human gene therapy</term><term>Lipofectin</term><term>Lower cytotoxicity</term><term>Mammalian cells</term><term>Mole</term><term>Molecular weight marker</term><term>Nacl buffer</term><term>Nucleic acids</term><term>Plasmid</term><term>Polyethylene glycol</term><term>Polymer</term><term>Polymer complexes</term><term>Reaction conditions</term><term>Retardation assay</term><term>Retroviral vectors</term><term>Room temperature</term><term>Size marker</term><term>Thionyl chloride</term><term>Tissue culture incubator</term><term>Transfection</term><term>Transfection assay</term><term>Transfection experiments</term><term>Transfection mixture</term><term>Uorescence intensity</term><term>Various amounts</term><term>Viral vectors</term><term>Weight ratio</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: A new series of gene carriers, polyethylene glycol (PEG)-grafted poly-l-lysine (PLL, mol. wt.=25 000) with three different PEG-grafted ratios (5, 10 and 25 mole%, which means 5, 10 and 25% of ϵ-amino group of PLL was modified by PEG), was synthesized. These new gene carriers, named comb-shaped PEG-g-PLL copolymer, showed a 5- to 30-fold increase in transfection efficiency compared to PLL alone on a human carcinoma cell line. It is likely that Hep G2 cells were transfected by plasmid DNA/PEG-g-PLL complexes through an endocytosis mechanism due to the fact that chloroquine increased transfection efficiency. Although Lipofectin™, a cationic lipid formulation, showed slightly higher transfection efficiency than PEG-g-PLL in Hep G2 cells, our designed PEG-g-PLL demonstrated lower cytotoxicity, early gene expression and maintenance of gene expression for up to 96 h.</div></front></TEI><affiliations><list><country><li>Corée du Sud</li><li>États-Unis</li></country><region><li>Région capitale de Séoul</li><li>Utah</li></region><settlement><li>Séoul</li></settlement><orgName><li>Université nationale de Séoul</li></orgName></list><tree><country name="Corée du Sud"><region name="Région capitale de Séoul"><name sortKey="Choi, Young Hun" sort="Choi, Young Hun" uniqKey="Choi Y" first="Young Hun" last="Choi">Young Hun Choi</name></region><name sortKey="Jong Sang Park" sort="Jong Sang Park" uniqKey="Jong Sang Park" first="" last="Jong Sang Park">Jong Sang Park</name></country><country name="États-Unis"><region name="Utah"><name sortKey="Liu, Feng" sort="Liu, Feng" uniqKey="Liu F" first="Feng" last="Liu">Feng Liu</name></region><name sortKey="Choi, Young Kweon" sort="Choi, Young Kweon" uniqKey="Choi Y" first="Young Kweon" last="Choi">Young Kweon Choi</name><name sortKey="Kim, Jin Seok" sort="Kim, Jin Seok" uniqKey="Kim J" first="Jin-Seok" last="Kim">Jin-Seok Kim</name><name sortKey="Kim, Sung Wan" sort="Kim, Sung Wan" uniqKey="Kim S" first="Sung Wan" last="Kim">Sung Wan Kim</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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